8/31/2023 0 Comments Biotechnology by r.c.dubey pdf![]() Since OD is directly proportional to the cell concentration (Dubey, 2014 Madigan et al., 2010), the concentration of each culture was estimated using the OD 600 and the concentration-OD 600 relationship for each indigenous microbe determined using methods discussed in section 2.4.Īfterward, around 0.1 mL of each culture was immediately added to 20 mL of phosphate buffered saline solution (PBS) and diluted to achieve the desired concentration of approximately 2 10 1 CFUmL of each microbe to reach a total concentration of 2 10 2 CFUmL before being added to the inoculation solution. The OD 600 of the resulting broth culture was measured and used to estimate the concentration of each culture. ![]() Advanced Biotechnology By Rc Dubey Free Public Fullĭubey Author content All content in this area was uploaded by R.C.ĭubey on Content may be subject to copyright.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |